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Samples were alkylated with 20 mM iodoacetamide (IOA) and incubated at room temperature for 1 h in the dark before the reaction was quenched with 2.5 mM dithiothreitol (DTT) and diluted to 0.1% SDC in 50 mM triethylammonium bicarbonate (TEAB) prior to proteolytic digest
Most patients report early changes (improved skin feel, reduced inflammation, or fewer shedding hairs) by the end of week 3 or 4
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