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The forward and reverse primers were mixed with 100 ng cDNA and quantitative RT-PCR was performed using PowerUp SYBR Green Master Mix (Applied Biosystems, Darmstadt, Germany) or THUNDERBIRD SYBR qPCR Mix (TOYOBO, Japan)

Specifically, 41.7% of the surveyed patients reported a noticeable benefit in managing their systemic fatigue, brain fog, and post-exertional malaise, highlighting its role as a highly accessible and effective management tool

Muoz-Pinedo C, Guo-Carrin A, Goldstein JC, Fitzgerald P, Newmeyer DD, Green DR
