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We've guided hundreds of researchers through this exact reconstitution sequence

TPO becomes active only after binding heme molecules, so its activity depends strictly on the iron status of the individual

Western blot results of recombinant SjGST20 and SjGST22
The C-terminal lysine carries an amide rather than a free carboxyl group, blocking carboxypeptidase cleavage of the C-terminus and protecting the C-terminal recognition motif required for full receptor activation