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Typical Post-Injection Timeline First 24-48 hours: Mild to moderate pain at the injection site Localized warmth and redness Slight swelling around the knee Temporary stiffness or reduced range of motion Days 2-3: Gradual reduction in swelling Decreased pain and tenderness Return to normal mobility Possible spongy sensation when walking [4] The American Academy of Orthopaedic Surgeons notes that patients should avoid excessive activity, such as jogging or heavy lifting for the first 48 hours after injection [2]

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After incubation, samples were directly analyzed by denaturing PAGE or proceeded to sequencing library preparation using the same method as described in the preceding sections

Increased firmness (weeks 6 to 10): lax areas, particularly the lower face, jawline, and neck, show improved tone as new collagen fibers are laid down