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To assess the status of RhoA and its activation in cardiac junctions, we employed the well-established TCA fixation method that allows for the visualization of junctional-specific endogenous RhoA by immunofluorescence (21, 22)

EMBO J 22:48154825

Moreover, based on the mounting evidence of an immune component in some individuals with ASD and the putative role of the GBA in its pathophysiology, anatomical (volume, weight, and uniformity), network, and functional alterations should be considered neither the principal cause nor necessarily fixed in ASD but rather a correlate of disease in the context of a multifactorial and multidimensional/multiscale aetiology and pathogenesis [113] along with multifactorial environmental insult in the setting of genetic and genomic vulnerability

M.StampferM