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The aqueous phase was mixed with equal volume of 70% ethanol, purified using the RNeasy mini kit (Qiagen), and final RNA concentration was calculated using NanoDrop (Thermo Fisher)
PMC8355064 Cationic amphiphilic compounds and human wheal-and-flare - Pharmacophore modeling and human skin-response evidence for MRGPRX2 activation
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The GPX8 gene is located on chromosome 5q11.2 and is composed of 4 exons that generate four alternatively spliced mRNAs
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Each sample was tested in two technical replicates
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