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Once reconstituted, the solution should be aliquoted and stored at 20C to preserve stability and avoid repeated freeze-thaw cycles, which can degrade peptide integrity

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It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the inhibition ratio is 10%~50% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment

The confocal microscopy images clearly depict the expression of respective fluorescence indicating the mode of repair mechanisms, which is in accordance with FACS analysis