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In HeLa cells, the Ag-NP-hydrogel exposure activated JAK-STAT signaling pathway by upregulating JAK-STAT cascade-related gene, thereby inducing toxicity (Xu et al., 2012)

For toxicity assays neuronal cell viability is typically monitored using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT), adenosine triphosphate (ATP), adenylate kinase, or lactate dehydrogenase (LDH) based cell viability assays, using neuronal or neuronal-like cells such as SHSY-5Y, PC12 cells, primary neurons, or more recently iPS cells from patient-derived fibroblasts (see [47] for an overview of cell death assays)