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To reduce the urea concentration, the samples were diluted 5-fold with ammonium bicarbonate (AMBIC) before overnight digestion at 37 C with trypsin (type IX-S from Porcine Pancreas) using a 1:30 (w/w) enzyme to substrate ratio

However, the main reason for combining the two substances is the improved stability of the L-citrulline, which allows it to be absorbed and utilized more effectively by the body

7B and Supplementary Figure S4)

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