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A widely cited inflection point came in 2023
The column was extensively washed with the wash buffer (50 mM HEPES-NaOH pH 8.0, 300 mM NaCl, 10% glycerol, 1 mM TCEP), and elution was performed with wash buffer containing 10 mM desthiobiotin

We selected variants corresponding to a depth 30x in the canonical isoform and a variant allele frequency (VAF) 0.3 and that were represented in at least 5% of our cohort
Aliabadi and Haney are the cohosts of She MD, a podcast that explores an array of womens health subjects and hosts influential guests
