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Data-independent acquisition (DIA) Proteomics assay Proteins were extracted using the SDT lysis buffer (4% SDS, 100 mM DTT, 100 mM Tris-HCl, pH 8.0) and then quantified
This completely bypasses the digestive system, avoiding the very absorption issues that tirzepatide creates

Instead, the CobaSorb test that measures change in serum holoTC concentrations following an oral dose of unlabeled B12 can be used

Subsequently, Sartini et al
