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Formigari A, Gregianin E, Irato P
In acid extracts, from which proteins have been precipitated, this method measures total non-protein thiols (Figure 2A, bottom)
To further explore this citrate method Bally et al., studied the effect of proton gradient by replacing the intraliposomal solution to HEPES buffer pH (7.0) from 300 mm citrate buffer (pH 4.0), keeping the internal pH undisturbed
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