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The effects of Hinge on synaptogenesis were evaluated by recording mEPSC frequencies on cultured hippocampal neurons

Glutamate release assays The Glutamate-Glo Assay kit (Promega, Madison, WI, USA) was used to quantify the amount of glutamate released into the conditioned medium

J-HC contributed to morphology analysis of GCLM -KO mice and editing the manuscript
The group-based multi-trajectory model (GBMT) and latent class analysis (LCA) were separately utilized to describe the distinct trajectories and patterns of pesticide mixture exposure during childhood