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Diameter, polydispersity, zeta potential and encapsulation efficiency of LNP-mRNA particles were determined using the NanoBrook Omni (Brookhaven), Zetaview (Particle Metrix, Germany) and Ribogreen assay (Thermo Fisher)

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In peptide research, variability can enter through inconsistent reconstitution assumptions, adsorption effects at low working concentrations, uncontrolled temperature excursions during preparation, and untracked freeze thaw events that shift effective delivered exposure
