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Among the cytokines, tumor necrosis factor (TNF) and IL-1 regulate leak pathway by promoting occludin endocytosis and MLCK activation [19, 23]

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To maintain the cyclic structure of the LyP-1 peptide as well as the CendR motif, DOTA was conjugated with the -NH 2 of the cysteine at the N-terminus of LyP-1 either directly or via the 6-aminocaproic acid (ACP) spacer (Fig

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