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Then, lysis buffer (1% NP-40, 10% Glycerol, 137 mM NaCl, 20 mM Tris pH7.6, 20 mM NaF, 2 g/mL aprotinin, 2 g/mL leupeptin, 2 g/mL pepstatin, 200 M Na3VO4, 1 mM PMSF) was added and cells were stored at 4 C for 30 min

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While some findings suggest that cannabinoids may have mood-stabilizing effects and enhance serotonin signaling, the evidence is inconclusive, and further research is warranted [61]