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Description
The entire pool of ~340,000 unique mutants was inoculated in LB medium at OD 600 0.025 (~75 CFU per mutant) and allowed to reach early exponential phase (OD 600 0.15)

doi: 10.18632/oncotarget.3849 124 BrownCWMercurioAM

The B12 injection goes into the muscle, which bypasses the entire GI system and is slowly absorbed by the blood over time circulating into the body in a more efficacious manner

Color changes: Persistent redness or darkening of the skin at injection sites suggests chronic irritation
