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Nutrients , 14 , 3566

To comprehensively elucidate BSP structures, we employed an integrated analytical platform: UVvisible spectroscopy, gas chromatography (GC), high-performance liquid chromatography (HPLC), size-exclusion chromatography (SEC, including high-performance gel permeation chromatography (HPGPC) variants), GCmass spectrometry (GC-MS), FT-IR spectroscopy, multinuclear NMR ( 1 H, 13C, and 2D), atomic force microscopy (AFM), scanning electron microscopy (SEM), and X-ray diffraction (XRD)

Key trials like STEP, SURPASS, and SELECT have demonstrated their efficacy in glycemic control (HbA1c reductions of 1.02.0%) [1, 3, 4], weight loss (1020% mean body weight reduction) [2, 3, 4], and cardiovascular risk reduction [4, 7]

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