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The SNX27-R68A mutant construct was generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S) with the forward primer 5- CGG GCA ACT GGC GAG CAT CAA CGG GGA G -3, and reverse primer 5- CCC TCG CTC ACT TGG CCC CGC ACG TTG AA -3
In intestinal inflammatory diseases, they contribute to the reduction of gut inflammation by preserving intestinal barrier integrity and modulating gut microbiota composition
Biomolecules 8 , 50 (2018)
