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Nat Commun 5:4904 Ramlackhansingh AF, Brooks DJ, Greenwood RJ, Bose SK, Turkheimer FE, Kinnunen KM, Gentleman S, Heckemann RA, Gunanayagam K, Gelosa G et al (2011) Inflammation after trauma: microglial activation and traumatic brain injury

Despite the importance of glutamate and NMDAR for the healthy brain, overactivation of glutamate receptors leads to toxic levels of intracellular calcium, which can result in cell death [133, 134], for which the term excitotoxicity was coined [135]

Ice formation, cryoconcentration, pH shifts, and repeated freeze-thaw cycles can damage peptide integrity or create aggregation

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