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Following the removal of the secondary antibody, the cells were fixed using an anti-fading fixing medium
In addition, the photoreceptor cells in diabetic mice also showed enhanced levels of iron, ROS, and MDA and reduced GSH concentration with downregulation of GPX4 and SLC7A11, and upregulation of ACSL4, FTH1 and NCOA4, which suggested that ferroptosis is also involved in the pathogenesis of photoreceptor degeneration in the development of the early stages of DR (Gao et al., 2023)
When the 1:5 ratio was administered, the acidosis was less marked, but still three of seven animals succumbed within 24 h
A key example is shown in the spectrum reported in Figure 4b for the conjugate of mercury (original, unpublished results from our laboratory)
