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Although contradictory results support that hypomethylation of SNCA intron 1 does not interfere with the transcription of the SNCA gene [248], a subsequent study using advanced technical approaches confirmed that the particular methylation is cell-type specific and indeed it regulates SNCA expression [249]

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In addition, other biological responses that decrease the potency of the drug product can occur 37

Such findings align with preclinical models showing that prolonged ethanol exposure induces persistent neuroadaptations in mood-regulating circuits, including the prefrontal cortex, hippocampus, and amygdala (Hasin and Grant 2015)