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The purified proteins were eluted using elution buffers containing reduced glutathione (10 mM) or imidazole (250 mM), respectively

doi: 10.1186/s12974-022-02400-6, [DOI] [PMC free article] [PubMed] [Google Scholar] 30.Yoshino H

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Early clinical trials indicated minimal activity, primarily because the drug couldnt penetrate the blood-brain barrier (BBB) effectively and because the tumor growth wasnt largely dependent on the targeted pathway ( Lapatinib, a dual inhibitor of EGFR and human epidermal growth factor receptor 2 (HER2), is the most frequently tested EGFR inhibitor in GB trials
