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1f, except it used either WT AGO2 (left panel) or a variant with mutations in the PAZ-domain pocket (F294A, Y311A) (right panel), and it used target RNAs with varying internal loop lengths (panel a ) (Supplementary Table 1)

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Alpha-ketoglutarate (-KG), a key metabolite in the tricarboxylic acid (TCA) cycle, serves as a substrate for TET enzymes, which are responsible for DNA demethylation (Chia et al

These two can start simultaneously NAD+ does not need to be loaded first