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Equal amounts of the extracted proteins were electrophoresed on SDS-PAGE gels and transmitted to a polyvinylidene fluoride membrane (ISEQ00010, Millipore), membrane was blocked (G2052, Servicebio) and incubated with primary antibodies overnight at 4C, including GAPDH (1:1000, 10494-1-AP, Proteintech), -tubulin (1:1000, 10094-1-AP, Proteintech), -actin (1:1000, ab8227, Abcam), GDF11 (MAB19625, Abnova), RUNX2 (1:1000, ab192256, Abcam), CD31 (1:500, GB11063-2, Servicebio), VEGFA (1:500, GB11034B, Servicebio), COL1 (1:500, ab279711, Abcam), AKT (1:500, #9272, Cell Signaling Technology), p-AKT (1:500, #4060, Cell Signaling Technology), PI3K (1:500, T40115, Abmart), p-PI3K (1:500, TA3242, Abmart), eNOS (1:1000, 27120-1-AP, Proteintech) and p-eNOE (1:500, PC3447, Abmart)

McCarty, Kieran

The process of regeneration is believed to occur either extrinsically by infiltration of external cells, intrinsically by tendon fibroblast proliferation, or both

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