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Techniques such as immunofluorescence microscopy and subcellular fractionation assist in pinpointing the subcellular position of transporters (167, 168), Furthermore, genetic manipulation techniques, including gene knockout and overexpression, allow for direct control over SLC7 transporter expression in cellular or animal models

Anesthesiology 132 , 11971211 (2020)

Given the difficulty of targeting endothelial cells in the dermis, inhibiting downstream pathways activating melanocytes, like EDN-1/EDN receptor signaling [176,177,178,179], could be particularly relevant for treating melasma and solar lentigo as this signaling pathway is upregulated in these conditions [180]
