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Safranin O staining Safranin O staining was performed to detect the changes in proteoglycans with a Safranin O staining kit (G1371, SolarBio) according to the manufacturers recommended procedure

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coli BL21(DE3) using standard IPTG induction and purified by Immobilized metal affinity chromatography (IMAC) followed by Size exclusion chromatography (SEC) using a Superdex75 16/60 column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.4, 150 mM NaCl, 5 % (v/v) glycerol

Because peptides target specific pathways, they are often used in mens health to address concerns such as slower recovery, reduced muscle mass, stubborn body fat, low energy, declining libido, and healthy aging