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If the solution, which should be perfectly clear, becomes cloudy, changes color, or has visible particulates, it should be discarded immediately as it may be contaminated or degraded
For imaging, samples were washed twice in dPBS1X and acquired with an Amnis ImageStreamX MkII Imaging Flow Cytometer using 60 magnification and ex/em wavelength of 642/690 nm to detect the locked nucleic acid signal
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174 , 18 (2018) A.T