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GABA measurement One gram of each powdered sprout sample was mixed with distilled water (1:5 w/v) at 150 rpm and 70 C for 90 min, then centrifuged (Kobuta, 4200, Japan) at 6000 g for 15 min
The peptide has been used to treat poor sleep, pain conditions, stress-related symptoms, and more
Estimation of Mitochondrial Concentration The concentration of isolated mitochondria was determined by conducting a protein assay using the Cytation 3 Cell Imaging Multi-mode Plate Reader (BioTek, Agilent, Switzerland) and the Take3 Microvolume plate
