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The supernatants were transferred to new microtubes and analyzed by western blot

Furthermore, prolonged Hcy treatment induced mitochondrial apoptosis of human umbilical vein endothelial cells through increased NADPH oxidase 4 (NOX4) expression and intracellular ROS production and decreased Bcl-2/Bax ratio and mitochondrial membrane potential (MMP), resulting in cytochrome c release and caspase-3 activation [69]
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2005;Mullooly et al
