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Risks from Concomitant Use with Benzodiazepines or Other CNS Depressants]

Without purification, the reaction mixture was treated with 7 equiv

The total reaction mixture consisting of 1.5 ml sodium phosphate buffer (0.1 M, pH 7.5) and PVP (1% w/v) L-methionine (13 mM), enzyme extract (0.1 ml) with same amounts of NBT solution (2.25 mM), riboflavin (60 M), Na 2 CO 3 (1 M), EDTA (3 mM) and double-distilled water (1.0 ml, DDW)

[5] Following Sanger's initial report of the reagent, the dinitrofluorobenzene method was widely adopted for studying proteins, until it was superseded by other reagents for terminal analysis (e.g., dansyl chloride and later aminopeptidases and carboxypeptidases) and other general methods for sequence determination (e.g., Edman degradation)