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ICP-MS measurements To quantify the possible presence and concentration of metal ions in GLT25D1/COLGALT1 recombinant preparations, 900 L of a sample containing 4 mg mL 1 GLT25D1/COLGALT1 (purified without usage of divalent ions) were treated with 300 L 65% ultrapure HNO 3 and 200 L 30% w/w H 2 O 2 , made up to 5 mL with ultrapure water and analyzed on a single quadrupole inductively coupled plasma mass spectrometer (SQ-ICP-MS, iCAP RQ Thermo Fisher Scientific), equipped with a quartz cyclonic chamber cooled at 3 C, a MicroMist nebulizer 179 (400 L min 1 ), a quartz torch, a Ni sampler, skimmer cones, and a QCell pressurized with helium (3 V, KED mode)

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