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For LC-MS, the reaction was prepared with 10 M enzyme, 200 M GTP in 20 mM HEPES buffer, pH 6.8, 150 mM NaCl, 10 mM MgCl 2 .The reaction was carried out at 30 C for 5 min, then deactivated at 95 C for 5 min
And as you said, what is effective versus symptoms is most important at the end of the day
Advisers to this series are Robin Ferner and Patricia McGettigan
Rampelt, H
