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To try to overcome this, she teamed up with Walenskys lab, which had previously shown that stapling these small peptides can make them more stable and help them get into cells
To purify uS10, the denatured ribosomes were incubated at 4 C with 50 L of pre-equilibrated Ni-NTA agarose (QIAGEN) for 1 h
While they can fade over time, it may take several months or even years for them to disappear completely
