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Anytime : For peptides with a long half-life or those whose action is not acutely dependent on nutrient timing (e.g., TB-500 , Thymosin Alpha-1)

CRBN contains a FLAG tag that binds to the AlphaLISA acceptor bead, which is conjugated with an anti-FLAG antibody

NMR spectra acquisition and assignment H NOESY spectra acquisition was conducted with a spectral width of 12 ppm, 20,000 data points, presaturation during the relaxation delay, and a mixing time for water suppression along with gradient spoilage, a 5-second relaxation delay, and a mixing time of 10 ms [31,32,33]

Glutathione reductase (GR) can use NADPH to catalyze the reduction of oxidized glutathione (GSSG) to GSH, eliminating LPO on the cell membrane and preventing cell membrane damage, thereby inhibiting ferroptosis (Yang et al., 2014)