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For imaging of live worm embryos (Nile Red, UV imaging, and fluorescently tagged constructs), embryos were resuspended in M9 buffer and dropped onto 3% agar pads
Daughter Caroline was born in Brooklyn in 1828 and, in 1829, also in Brooklyn, Sarah Lang was born, but died at the age of 15 months on May 17, 1830, according to a New York Evening Post announcement of her death
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