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10.1136/thoraxjnl-2019-213738 19 BuddeJ.SklootG
The pretraining comprised of four sessions on three successive days adopting an accelerating protocol that began at 5 rpm and increased to 20 rpm in 5 min
In vivo yeast expression assays of the LjFNSII and LmFNSII proteins To investigate the catalytic activity of the enzymes encoded by the isolated putative FNSII genes, the coding region of each sequence was inserted into the pYeDP60 vector 25 and transformed into the WAT11 yeast strain, which expresses the Arabidopsis NADPH-cytochrome P450 reductase 26 and provides the reducing equivalents indispensable for the activity of plant CYP450s such as flavone synthase II enzymes
The advantage of this method is that it can detect oxidative stress levels in real time
