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J Clin Virol Off Publ Pan Am Soc Clin Virol
10.1016/S2352-4642(22)00314-5 65 LeeS.LeeH
Experiments were performed at room temperature with glass pipettes (45 M resistance) filled with the intracellular solution comprising 140 mM KGluconate, 4 mM NaCl, 1 mM MgCl 2 , 1 mM EGTA, 5 mM HEPES, 2 mM MgATP, 0.6 mM NaGTP, pH = 7.4 (KOH)
The analysis revealed a prominent decrease of m 6 A modification in mRNA from RBM15 KO and RBM15 SPOC, whereas SPOC deletion in SHARP did not have a pronounced effect (Fig
