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Description
High-throughput Measurement of Binding Kinetics by MRNA Display and Next-generation Sequencing
Wang X, Zhou Y, Min J, Wang F
Briefly, metabolites in plasma (100 L) were extracted with 90% methanol (900 L), and proteins were precipitated at -20 C for 2 h, followed by centrifugation for 10 min at 4000 rpm at 4 C
Rather than injecting large doses infrequently (most of which you will flush out within an hour), its much better to inject smaller, daily doses
