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qPCR reactions were performed with FastSYBR Green Master Mix (ThermoFisher) plus 10 ng of cDNA in a final volume of 10 L
It is difficult to determine whether or not a certain gene is off targeted in this case

Rate of utilization of glucose and compartmentation of -oxoglutarate and glutamate in rat brain
doi: 10.1164/ajrccm.160.3.9811018 221 StojiljkovicI.KumarV.SrinivasanN