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Significance was determined by one-way repeated measures analysis of variance, and the HolmSidak method for multiple comparisons versus the control group, using SigmaPlot 10.0 software (Systat Software, San Jose, CA, USA)

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Delta sleep-inducing peptide (DSIP) is composed of 9 amino acids and has been implicated in the regulation of sleep, modulation of stress ( Pichia pastoris , emerges as a promising candidate in this regard, with its sedative and hypnotic effects garnering significant interest